An immobilized enzyme being used in a continuous plug flow reactor exhibits an effectiveness factor (η) of 1.2. The value of η being greater than 1 could be apparently due to
(A) substrate inhibited kinetics with external pore diffusion limitation
(B) internal pore diffusion limitation
(C) sigmoidal kinetics
(D) unstability of the enzyme

1 Answer
Determine the correctness or otherwise of the following Assertion (a) and the Reason (r):
Assertion: Somatic embryogenesis in plants is a two-step process comprising of embryo initiation followed by embryo production.
Reason: Embryo initiation is independent of the presence of 2,4-dichlorophenoxyacetic acid whereas embryo production requires a high concentration of 2,4-dichlorophenoxyacetic acid.
(A) both (a) and (r) are true and (r) is the correct reason for (a)
(B) both (a) and (r) are true and (r) is not the correct reason for (a)
(C) (a) is true but (r) is false
(D) (a) is false but (r) is true

1 Answer
The formation of peptide cross-links between adjacent glycan chains in cell wall synthesis is called
(A) Transglycosylation
(B) Autoglycosylation
(C) Autopeptidation
(D) Transpeptidation

1 Answer
Nuclease-hypersensitive sites in the chromosomes are sites that appear to be
(A) H2 and H4 histone free
(B) H1 and H2 histone free
(C) H3 and H4 histone free
(D) Nucleosome free

1 Answer
Oxidation-reduction reactions with positive standard redox potential (E⁰’) have
(A) positive ΔG⁰
(B) negative ΔG⁰
(C) positive ΔE⁰’
(D) negative ΔE⁰’

1 Answer
The degree of inhibition for an enzyme-catalyzed reaction at a particular inhibitor concentration is independent of the initial substrate concentration. The inhibition follows
(A) competitive inhibition
(B) mixed inhibition
(C) un-competitive inhibition
(D) non-competitive inhibition

1 Answer
Identify the enzyme that catalyzes the following reaction:
α-Ketoglutarate + NADH + NH4+ + H+ → Glutamate + NAD+ + H2O
(A) Glutamate synthetase
(B) Glutamate oxoglutarate aminotransferase
(C) Glutamate dehydrogenase
(D) α-ketoglutarate deaminase

1 Answer
During lactic acid fermentation, net yield of ATP and NADH per mole of glucose is
(A) 2 ATP and 2 NADH
(B) 2 ATP and 0 NADH
(C) 4 ATP and 2 NADH
(D) 4 ATP and 0 NADH

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In a chemostat operating under steady state, a bacterial culture can be grown at a dilution rate higher than maximum growth rate by
(A) partial cell recycling
(B) using sub-optimal temperature
(C) pH cycling
(D) substrate feed rate cycling

1 Answer
Expressed Sequence Tag is defined as
(A) a partial sequence of a codon randomly selected from cDNA library
(B) the characteristic gene expressed in the cell
(C) the protein coding DNA sequence of a gene
(D) uncharacterized fragment of DNA present in the cell

1 Answer