Questions and Answers

State that chlorophyll is a green pigment that is found in chloroplasts

Explain the effect of changes in temperature and pH on enzyme activity in terms of kinetic energy, shape and fit, denaturation and the frequency of effective collisions

Investigate and describe the effects of temperature and pH on enzyme activity

Describe that the progress of enzyme-catalysed reactions can be followed by measuring the concentrations of reactants and products

Explain the specificity of enzymes in terms of the complementary shape and fit of the active site with the substrate (‘lock and key’ hypothesis)

Explain enzyme action with reference to the substrate, active site, enzyme-substrate complex, and product

Describe enzymes as proteins that function as biological catalysts and are involved in all metabolic reactions

Describe a catalyst as a substance that increases the rate of a chemical reaction and is not changed by the reaction

Describe and be able to do chemical tests for: (a) starch (iodine solution) (b) glucose and maltose (Benedict’s solution) (c) protein (biuret test) (d) lipids (ethanol emulsion test)

State that large molecules are made from smaller molecules, limited to: starch, cellulose and glycogen from glucose; proteins from amino acids; lipids from fatty acids and glycerol; DNA from nucleotides