Explain that gene editing is a form of genetic engineering involving the insertion, deletion or replacement of DNA at specific sites in the genome

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Explain how gene expression may be confirmed by the use of marker genes coding for fluorescent products

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Explain why a promoter may have to be transferred into an organism as well as the desired gene

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Explain the roles of restriction endonucleases, DNA ligase, plasmids, DNA polymerase and reverse transcriptase in the transfer of a gene into an organism

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Explain that genes to be transferred into an organism may be: • extracted from the DNA of a donor organism • synthesised from the mRNA of a donor organism • synthesised chemically from nucleotides

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Explain that genetic engineering is the deliberate manipulation of genetic material to modify specific characteristics of an organism and that this may involve transferring a gene into an organism so that the gene is expressed

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Outline the role in conservation of the International Union for Conservation of Nature (IUCN) and the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)

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Explain reasons for controlling invasive alien species

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Describe methods of assisted reproduction used in the conservation of endangered mammals, limited to IVF, embryo transfer and surrogacy

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Outline the roles of zoos, botanic gardens, conserved areas (including national parks and marine parks), ‘frozen zoos’ and seed banks, in the conservation of endangered species

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