can two dimensional polyacrylamide gel electrophoresis determine 3d

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is used to separate proteins based on two different properties, typically isoelectric point (first dimension) and molecular weight (second dimension). However, it does not determine the three-dimensional structure of proteins. For 3D structural analysis, techniques such as X-ray crystallography or nuclear magnetic resonance (NMR) spectroscopy are required.