Comparison Between Kinyoun Stain vs Ziehl neelsen stain

Published on May 20, 2024 By BNO Team Fact checked & reviewed by BNO Team

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Kinyoun stain vs Ziehl neelsen Stain

Characteristics	Kinyoun stain	Ziehl Neelsen Stain
Primary Stain	In Kinyoun stain method basic fuchsin is used as a primary stain.	In Ziehl Neelsen Stain method, carbol fuchsin is used as a primary stain.
Phenol concentration	A high concentration of Phenol is used.	A low concentration of phenol is used.
Heating	During the staining procedure cells are not heated.	Cells are heated for 5 minutes during the staining procedure.
Concentration of Primary stain	A high concentration of primary stain is used.	A low concentration of primary stain is used as compared to kinyoun stain.
Mordant	High concentration of Phenol and basic fuchsin act as a mordant and help in the penetration of stain into the cell.	Phenol and heat act as a mordant and help in penetration of stain into the cell.
Decolorization	The primary stain is not removed by the acid-alcohol.	The primary stain is removed by the decolorizer.
Toxic effect	Kinyoun stain method is safe as compared to the Ziehl Neelsen Stain method because in this method the phenol is not heated.	In Ziehl Neelsen Stain method phenol is heated as a result it giving rise to toxic form that can harm our eye and mucus membrane.

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