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Comparison Between Kinyoun Stain vs Ziehl neelsen stain

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Kinyoun stain vs Ziehl neelsen Stain

CharacteristicsKinyoun stainZiehl Neelsen Stain
Primary StainIn Kinyoun stain method basic fuchsin is used as a primary stain.In Ziehl Neelsen Stain method, carbol fuchsin is used as a primary stain.
Phenol concentrationA high concentration of Phenol is used.A low concentration of phenol is used.
HeatingDuring the staining procedure cells are not heated.Cells are heated for 5 minutes during the staining procedure.
Concentration of Primary stainA high concentration of primary stain is used.A low concentration of primary stain is used as compared to kinyoun stain.
MordantHigh concentration of Phenol and basic fuchsin act as a mordant and help in the penetration of stain into the cell.Phenol and heat act as a mordant and help in penetration of stain into the cell.
DecolorizationThe primary stain is not removed by the acid-alcohol.The primary stain is removed by the decolorizer.
Toxic effectKinyoun stain method is safe as compared to the Ziehl Neelsen Stain method because in this method the phenol is not heated.In Ziehl Neelsen Stain method phenol is heated as a result it giving rise to toxic form that can harm our eye and mucus membrane.

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