Blood Cross Matching – Principle, Procedure, Result.
Cross Match is a laboratory test, performed prior to a blood transfusion to determine whether donor blood is compatible (or incompatible) with recipient blood.
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Blood Grouping Test – Principle, Procedure, Result
The concept of Blood grouping was first discovered by Karl Landsteiner in 1901, who was an Austrian-American immunologist and pathologist. He received Nobel Prize in Physiology or Medicine in 1930 for this discovery.
Gel Staining Procedure for PAGE
Staining protein gels using Coomassie Brilliant Blue R-250 is common to see proteins that are resolved using SDS-PAGE. It is extremely sensitive and suitable for long-term storage of gels.
Laboratory Rules – Basic Rules Of A Microbiology Laboratory
A well-established microbiology laboratory is necessary for the work of microorganisms. The students/researchers/scientists must follow the basic rules while working with microorganisms.
Measurement Of Microorganisms – Micrometry
A microscope comes with an objective of 10X (low power) 45X (high power) and 100X (oil immersion). and the ocular lens with 5X, 10X and 15X. In general, an 10-X ocular lens is used in most cases. If an animal can be clearly seen with 10X ocular lens and 45X objective, this means that the size is 10×45, which is 450 times larger than the standard one. We don’t know the exact measurement is ? It could be determined using an calibrated scale. Thus the calibration of all lenses (eyepieces) is performed.
Giolitti-Cantoni Broth for Staphylococcus aureus
Giolitti Cantoni Broth Base with the addition of potassium tellurite is utilized to enrich selectively Staphylococcus aureus, a food-borne pathogen, from suspected items, according to ISO.
Eastern blot – Protocol, Principle, Application
A Molecular Biology Technique is used for the identification of Post Translation Modification mostly carbohydrate Epitopes by transferring onto a membrane and analyzed using a probe.
Dot Blot – Protocol, Principle, Definition.
Dot Blot is a simplified technique of western blotting, which is mainly used for the detection of proteins.
Restriction Digest – Protocol, Principle, result
The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.
Reverse Blood Grouping – Principle, Procedure, Result
Reverse grouping is defined as using the reagent cells with known ABO antigens and testing the patient’s serum for ABO group antibodies.