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Ninhydrin Test Principle, Procedure, Result, Uses

Ninhydrin Test Principle, Procedure, Result, Uses

What is the Ninhydrin Test? The ninhydrin chemical test is used for determining whether an analyte contains any amines or amino acids. In this test, ninhydrin (a chemical compound with the formula C9H6O4; IUPAC name: 2,2-dihydroxyindane-1,3-dione) is added to a test solution of the analyte. The formation of a deep blue color within the test … Read more

Phenylalanine Deaminase Test Principle, Procedure, Result

Phenylalanine Deaminase Test Principle, Procedure, Result

Objective of Phenylalanine Deaminase Test Principle of Phenylalanine Deaminase Test Requirements For the test Composition of Phenylalanine agar DL-Phenylalanine: 2 gm Yeast extract: 3 gm Sodium chloride: 5 gm Disodium phosphate: 1 gm Agar: 12 gm Distilled water: 1000 ml pH: 7.3 Preparation of Phenylalanine agar slant Note: Following autoclaving, the medium should appear lighter … Read more

Peroxide Value Test Principle, Procedure, Result

Peroxide Value Test Principle, Procedure, Result

Unsaturated fish oils are especially vulnerable to oxidation, resulting in peroxides when stored in cold or freezing conditions for storage. Peroxides are precursors to breakdown products that can cause rancid taste in fat. Peroxide levels are an indicator of oxidation in the initial stages of lipid degradation. The index is less reliable in the latter … Read more

Nelson Somogyi Method for Determination of reducing sugars

Nelson Somogyi Method for Determination of reducing sugars

Nelson-Somogyi method can be used to quantify the reduction of sugar using arsenolmolibdat and copper reagents. The principle behind the Nelson Somogyi method is the quantity of deposro oxide deposits which react with arsenomolibdate, which reduces to molybdine blue. The blue hue is determined by as absorbance. The keto and aldehyde free groups are regularly … Read more

Top 15 Books for Molecular Biology

Top 15 Books for Molecular Biology

In this article, I will share a list of Top and Best Molecular Biology Books. In this paper on microbiology, you will study about Structures of DNA and RNA / Genetic Material,  Replication of DNA (Prokaryotes and Eukaryotes), Transcription in Prokaryotes and Eukaryotes, Post-Transcriptional Processing, Translation (Prokaryotes and Eukaryotes), Regulation of gene Expression in Prokaryotes … Read more

Total White Blood Cell (WBC) Count – Total Leucocyte Count (TLC)

Total White Blood Cell (WBC) Count - Total Leucocyte Count (TLC)

The White Blood Cells (WBCs) also known as Leucocytes are round nucleated cells in blood, that provide protection against foreign invaders. There are many types of White Blood cells found in blood, and identified as Granulocytes (Neutrophils, Eosinophils & Basophils) and Agranulocytes (Monocytes and Lymphocytes). The size of White Blood Cells can vary between 10 … Read more

Centrifuge Blood Sample – Purpose, Steps, Techniques

Centrifuge Blood Sample - Purpose, Steps, Techniques

What are the components of blood? Blood is a fluid tissue that circulates throughout the body and performs a number of important functions. It is made up of several components, including cells and plasma. Cells: Plasma: Plasma is the liquid portion of the blood that carries the cells and other substances throughout the body. It … Read more

Reticulocyte Count – Principle, Procedure, Calculations and Clinical Significances

Reticulocyte Count : Principle, Procedure, Calculations and Clinical Significances

What is Reticulocytes? Reticulocytes are immature red blood cells (erythrocytes) that have not yet completed the final stages of maturation. They are usually produced in the bone marrow and have a characteristic reticulated (network-like) appearance when stained with special dyes. Reticulocytes are important in the evaluation of anemia, as they provide information about the bone … Read more

Total Red Blood Cell (RBC) Count – Procedure, Principle, result

Total Red Blood Cell (RBC) Count - Procedure, Principle, Result

Haemocytometry is a quantitative method that quantifies the RBC count in blood samples. The manual method of counting RBCs is the Haemocytometer (or Neubauer’s chamber slides). Modern devices, such as photometric and electrometric counters, can be more precise or automated. They can count the cells in the blood sample. It is impossible to count the … Read more

Sahli’s Method For The Estimation Of Hemoglobin

Sahli’s Method For The Estimation Of Hemoglobin

Hemoglobin, often abbreviated as Hb, is a vital protein found in red blood cells (RBCs). Its primary role in the human body includes the transport of oxygen from the lungs to various tissues and the transfer of carbon dioxide from tissues back to the lungs. Additionally, hemoglobin plays a significant role in maintaining the pH … Read more

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