BNO Team 
11 min read 
Because the aim of biologists is to cultivate microorganisms or eukaryotic cells with no introduction of any extraneous organisms aseptic methods are essential for ensuring that experiments are conducted with precision and accuracy. Always keep in mind that a totally safe working environment for scientists does not exist.
5 min read 
Spread Plate Method in microbiology The spread plate method is used to isolate individual colonies from a diluted sample of mix population. Different methods have been developed for the isolation of microorganisms from a mixture sample but among them Spread plate culture method is the most widely used technique in laboratory. An ideal spread plate … Read more
14 min read 
The pour Plate Method technique was created in the laboratory by Robert Koch and is still widely used in the laboratory since his time. This technique is appropriate for Microaerophilic, facultative, or anaerobic microorganisms. It is straightforward to use, requires fewer resources, and is simple and cost-effective. However, it requires the specimen to be placed … Read more
5 min read 
Overview of Subculturing Technique In microbiology, subculture is defined as a new cell or microbiological culture, which is prepared by transferring a few or all cells to a fresh growth medium from an old culture or a previous culture. In a single word, the transfer of cells from a previous culture to a fresh or … Read more
4 min read 
Aim Transfer of microbial cells from a parent culture medium to a fresh broth culture. Principle The broth is a liquid medium, which is used to grown microorganisms within the laboratory on a small scale. It also used in an industrial fermentor on a large scale. The media called broths, milks, or nutrient solutions are … Read more
11 min read 
Streak Plate Method Overview There are two types of microbial culture such as mixed culture and pure culture. The mixed culture contains two or more species while the pure culture contains only a single species. During the identification of a microorganism, the first and important step is to isolate the individual species from a mixed … Read more
8 min read 
Glucose, a key sugar molecule, plays a vital role in the human body as the primary carbohydrate found in the bloodstream. Its importance lies in its function as the main source of energy for various body tissues, notably the brain. When glucose is metabolized, it undergoes a sequence of chemical processes that result in the … Read more
8 min read 
What is Folin-Wu Method? Principle of Folin-Wu Method Requirements Procedure of Folin-Wu Method Step 1: Preparation of Protein-Free Filtrate Step 2: Testing Procedure This process results in a color change proportional to the glucose concentration in the blood sample. By comparing the absorbance of the test sample against the standard, the glucose level in the … Read more
9 min read 
What is Lane-Eynon Method? Principle of Lane-Eynon Method The Lane-Eynon method is a titrimetric procedure that quantifies reducing sugars in a solution through the utilization of Fehling’s solution as the primary reagent. This method operates on the fundamental chemistry of reduction-oxidation reactions, where Fehling’s solution, composed of copper sulfate and an alkaline solution of sodium … Read more
7 min read 
Milk is widely recognized as a significant source of calcium, an essential mineral that plays a crucial role in the development and maintenance of strong bones and teeth. This mineral is particularly important for infants and young children, whose rapidly growing bodies demand high levels of calcium to ensure proper bone formation and density. Calcium’s … Read more