Modified Thayer Martin Agar Composition

Thayer and Martin have reported improvement of the Chocolate Agar formulation that contained antimicrobics vancomycin and colistin, Nystatin. These ingredients were added to limit the growth of contaminants, inhibit the development of the saprophytic Neisseria sp. and to enhance the development of the pathogenic Neisseria. Martin and Lester have added an antibiotic, trimethoprim, which made them more selective. They also demonstrated to be effective in the fight against Proteus spp. Trimethoprim lactate refrains Proteus swarming. The resultant medium is known as Modified Thayer Martin. It’s a suitable and rich medium to isolate as well as cultivation of the Neisseria species. from mixed flora and suppression of other Gram-negative diplococci and gram-negative bacilli yeast and other gram-positive organisms.