Brain Heart Infusion (BHI) Agar – Composition, Principle, Preparation, Results, Uses
12 min read 
Rosenow developed a medium that could be used to cultivate streptococci using a dextrose broth and brain tissue in 1919. Hayden modified Rosenow’s formula and found that crushed marble promoted the growth of dental pathogens. The current formulation uses infusions from calf brain instead of brain tissue, and disodiumphosphate has been replaced by calcium carbonate.