SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe

SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe

SSPE (Blot, 20x, pH 7.4) Preparation and Recipe Required components Component Amount Concentration Sodium chloride (mw: 58.44 g/mol) 175.3 g 3 M Sodium Phosphate Monobasic Monohydrate (mw: 137.99 g/mol) 27.6 g 0.2 M EDTA (mw: 292.24 g/mol) 7.4 g 0.02 M SSPE (Blot, 20x, pH 7.4) Preparation Prepare 800 mL of distilled water in a suitable container. Add 175.3 g of Sodium chloride to the solution. Add 27.6 g of Sodium Phosphate Monobasic Monohydrate to the solution. Add 7.4 g of EDTA to the solution. Add distilled water until the volume is 1 L. … Read more

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