Molecular biology 1 Views 1 Answers
You synthesized a new peptide in your lab, which is permeable to the cell membrane. When you added it to eukaryotic cells, you noticed that the cells do not divide and eventually die. Upon further analysis, you discovered that a small replication bubble forms but does not last long, and the strands come back together. What molecules do you think are affected in your cells? – A. Lysosome – B. Polymerase I – C. Polymerase III – D. Topoisomerase – E. Single-stranded binding proteins (SSBP)
You synthesized a new peptide in your lab, which is permeable to the cell membrane. When you added it to eukaryotic cells, you noticed that the cells do not divide and eventually die. Upon further analysis, you discovered that a small replication bubble forms but does not last long, and the strands come back together. What molecules do you think are affected in your cells?
– A. Lysosome
– B. Polymerase I
– C. Polymerase III
– D. Topoisomerase
– E. Single-stranded binding proteins (SSBP)
Answered
E. Single-stranded binding proteins (SSBP) are likely affected. These proteins stabilize single-stranded DNA during replication, preventing the strands from reannealing. If inhibited, replication bubbles collapse, halting DNA replication.
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