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Sourav PanNovember 7, 2024

Why must tissues or organs used in medical procedures be bathed in a solution with the same osmolarity as the cytoplasm to prevent osmosis?

Why must tissues or organs used in medical procedures be bathed in a solution with the same osmolarity as the cytoplasm to prevent osmosis?

Sourav Pan
Sourav PanNovember 7, 2024

Answered

Tissues or organs used in medical procedures must be bathed in a solution with the same osmolarity as the cytoplasm to prevent osmosis, which can lead to cellular damage. Here’s why maintaining isotonic conditions is critical:

Osmosis and Cell Integrity

Osmosis is the movement of water across a semipermeable membrane from an area of lower solute concentration (hypotonic) to an area of higher solute concentration (hypertonic). When tissues are placed in solutions with different osmolarities than their cytoplasm, several outcomes can occur:

  • Hypotonic Solutions: If tissues are exposed to a hypotonic solution (lower osmolarity than the cytoplasm), water will enter the cells to balance solute concentrations. This influx can cause cells to swell and potentially burst, leading to cell lysis and tissue damage.
  • Hypertonic Solutions: Conversely, if tissues are placed in a hypertonic solution (higher osmolarity), water will leave the cells, causing them to shrink or crenate. This dehydration can disrupt cellular functions and lead to necrosis.

Importance of Isotonic Solutions

To maintain cellular integrity during medical procedures, it is essential that the bathing solution is isotonic—having the same osmolarity as the cytoplasm (approximately 286 mOsmoles/L). This ensures that:

  • No Net Water Movement: An isotonic environment prevents net movement of water into or out of cells, maintaining their normal shape and function.
  • Cell Viability: By preventing osmotic stress, tissues remain viable for transplantation or surgical procedures, reducing the risk of complications related to cell damage.

Clinical Relevance

In clinical settings, isotonic solutions such as saline (0.9% NaCl) are commonly used for rinsing tissues or during surgeries. This practice helps preserve cell function and viability, ensuring better outcomes for patients undergoing medical interventions

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