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SouravSeptember 18, 2024

Which statement about high-throughput DNA sequencing is false?

Which statement about high-throughput DNA sequencing is false?

Sourav
SouravSeptember 18, 2024

Answer

A false statement about high-throughput DNA sequencing is that it requires the use of radioactive labels to detect DNA sequences. In reality, modern high-throughput sequencing methods typically use fluorescent dyes or other non-radioactive detection methods. For example, sequencing by synthesis uses fluorescently labeled nucleotides that emit light when incorporated into a growing DNA strand. These fluorescent signals are then captured and analyzed to determine the DNA sequence. The use of non-radioactive methods not only makes the process safer but also more compatible with high-throughput automation and analysis.

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