Biochemistry 8 Views 1 Answers
Which one of the following is the correct match between the molecular properties listed in Column I and the corresponding biochemical separation methods in Column II?
Which one of the following is the correct match between the molecular properties listed in Column I and the corresponding biochemical separation methods in Column II?
| Column I | Column II |
|---|---|
| P. Solubility | i. Reverse phase chromatography |
| Q. Ionic charge | ii. Ultracentrifugation |
| R. Polarity | iii. Salting out |
| S. Molecular size | iv. Isoelectric focusing |
| v. Gel electrophoresis |
(A) P-i; Q-ii; R-v; S-iii
(B) P-iii; Q-iv; R-ii; S-i
(C) P-iii; Q-iv; R-i; S-ii
(D) P-v; Q-iv; R-iii; S-ii
Answered
Answer: (C) P-iii; Q-iv; R-i; S-ii
Explanation:
- Solubility → Salting out
- Ionic charge → Isoelectric focusing
- Polarity → Reverse phase chromatography
- Molecular size → Ultracentrifugation
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