Which of the following is a mechanism used in next generation high throughput DNA sequencing?
Which of the following is a mechanism used in next generation high throughput DNA sequencing?
Answer
Several mechanisms are used in next-generation high-throughput DNA sequencing, including: 1) Sequencing by synthesis (SBS): This mechanism involves the incorporation of fluorescently labeled nucleotides into a growing DNA strand, with each nucleotide addition being detected by imaging systems. 2) Sequencing by ligation: This method uses labeled oligonucleotides that are ligated to the DNA fragments, and the sequence is determined based on the emitted fluorescence. 3) Ion semiconductor sequencing: This technique measures changes in pH as nucleotides are incorporated into the DNA strand, with each nucleotide addition resulting in a detectable pH change. 4) Single-molecule real-time (SMRT) sequencing: This method, used by PacBio, sequences DNA by observing the synthesis of individual DNA molecules in real-time. Each mechanism offers unique advantages and contributes to the high-throughput capabilities of modern sequencing technologies.
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