Which of the following approaches is not used to create a transgenic animal?
Which of the following approaches is not used to create a transgenic animal?
Pronuclear microinjection
Retrovirus-mediated transgenesis
Sperm-mediated DNA transfer
Embryo twinning
Transfection of ES cells
Answer
The approach that is not used to create a transgenic animal is:
Embryo twinning.
Embryo twinning refers to the process of splitting an embryo to create genetically identical offspring and does not involve the introduction of foreign genes, which is essential for creating transgenic animals. The other methods listed are all techniques used to introduce new genetic material into an organism.