Which is part of a DNA sequencing technique that is useful for high-throughput sequencing, but is not part of Sanger DNA sequencing?

Sourav PanSeptember 18, 2024

Answered

The correct option is:

c. Labeling deoxynucleotides with fluorescent tags

Explanation:

• a. Using dideoxynucleotides as chain terminators: This technique is specific to Sanger sequencing, where dideoxynucleotides are used to terminate DNA chain elongation, generating fragments of varying lengths for sequence determination.
• b. Removal of a chemical group that blocks the 3′ end of the new DNA strand: This is part of some high-throughput sequencing technologies but not specific to the general concept of high-throughput sequencing as a distinguishing feature.
• c. Labeling deoxynucleotides with fluorescent tags: This is a feature of high-throughput sequencing technologies, such as Illumina sequencing. Fluorescently labeled nucleotides are used to detect the incorporation of each nucleotide during sequencing-by-synthesis, which is not part of the Sanger sequencing method.
• d. Separating DNA by size using gel electrophoresis: This technique is used in Sanger sequencing to separate DNA fragments, but high-throughput sequencing methods often use different approaches, such as optical detection and sequencing-by-synthesis.
• e. Hybridization between the template and a primer: Hybridization is used in both Sanger and high-throughput sequencing techniques to initiate the sequencing process. It is not a unique feature distinguishing high-throughput sequencing from Sanger sequencing.