Where in the cell does DNA replication take place?

DNA replication takes place in the nucleus of eukaryotic cells and in the cytoplasm of prokaryotic cells. In eukaryotes, the DNA is organized into chromosomes within the nucleus, and replication begins at specific origins of replication on each chromosome. In prokaryotic cells, the DNA is in the form of a single circular chromosome, and replication starts at the origin of replication within the cytoplasm.