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The Krebs cycle takes place in the mitochondrial matrix of eukaryotic cells, a specialized compartment inside mitochondria containing all the enzymes required for the cycle’s reactions. In prokaryotic cells, the cycle occurs in the cytoplasm, as these cells lack membrane-bound organelles. This localization in eukaryotes allows efficient interaction with the electron transport chain located in the inner mitochondrial membrane, optimizing the transfer of high-energy electrons produced in the cycle.
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