When using recent high-throughput sequencing methods, the genome assembly relies on

Recent high-throughput sequencing methods rely heavily on computational algorithms and bioinformatics tools for genome assembly. The sequencing process generates millions of short DNA fragments, and these fragments need to be accurately assembled into a complete genome sequence. Specialized software and algorithms, such as de Bruijn graphs, overlap-layout-consensus methods, and reference-based assembly approaches, are used to piece together these short reads into longer contiguous sequences. The assembly process also involves error correction, alignment to reference genomes, and statistical analysis to ensure the accuracy and completeness of the final genome assembly.