What is the first step in producing a transgenic animal?
What is the first step in producing a transgenic animal?
Answer
The initial step in producing a transgenic animal involves the construction of a transgene, which is a DNA sequence that consists of three main components: a promoter, the gene of interest, and a termination sequence. The promoter is a regulatory element that drives the expression of the gene, ensuring it is activated in the appropriate tissues or at specific stages of development. The gene of interest is the specific sequence that will provide the new trait or function once expressed. Finally, the termination sequence signals the end of transcription, ensuring proper regulation of gene expression. This carefully designed transgene forms the basis for subsequent genetic manipulation processes.
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