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In glycolysis, NADH is produced during the oxidation of glyceraldehyde-3-phosphate (G3P) to 1,3-bisphosphoglycerate. NAD+ accepts electrons from G3P, becoming reduced to NADH. NADH carries these high-energy electrons to the electron transport chain (in aerobic conditions) or to fermentation pathways (under anaerobic conditions) to help regenerate NAD+ and sustain glycolysis.
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