Identify the CORRECT statements: P. 5′ and 3′ ends of the transcripts can be mapped by utilizing polymerase chain reaction Q. S1 nuclease can cleave the DNA strand of a DNA-RNA hybrid R. T4 polynucleotide kinase is used for labeling 3′ end of DNA S. Baculovirus (Autographa californica) can be used as an insect expression vector Options: (A) P and Q only (B) R and S only (C) P and S only (D) Q and R only
Identify the CORRECT statements:
P. 5′ and 3′ ends of the transcripts can be mapped by utilizing polymerase chain reaction
Q. S1 nuclease can cleave the DNA strand of a DNA-RNA hybrid
R. T4 polynucleotide kinase is used for labeling 3′ end of DNA
S. Baculovirus (Autographa californica) can be used as an insect expression vector
Options:
(A) P and Q only
(B) R and S only
(C) P and S only
(D) Q and R only
Answer
Answer: (C) P and S only
Explanation: 5′ and 3′ ends of transcripts can be mapped using various PCR methods (P), and Baculovirus is used as an insect expression vector (S). S1 nuclease cleaves single-stranded regions in DNA-RNA hybrids, not the DNA strand (Q), and T4 polynucleotide kinase is used for labeling the 5′ end of DNA, not the 3′ end (R).
Sourav
