how to read polyacrylamide gel electrophoresis results

Sourav PanFebruary 9, 2025

Answered

To interpret PAGE results, researchers stain the gel with dyes such as Coomassie Brilliant Blue or silver stain to visualize separated bands. The position of the bands corresponds to molecular weight, with smaller molecules traveling farther than larger ones. A molecular weight marker (ladder) is often run alongside samples for reference. The intensity of bands can indicate protein concentration, and additional techniques like Western blotting can further analyze specific proteins.