how to prepare polyacrylamide gel electrophoresis

Preparing a PAGE gel involves mixing acrylamide, bis-acrylamide, buffer solution, and a polymerizing agent (e.g., TEMED and ammonium persulfate) to initiate gel formation. The solution is poured into a gel casting apparatus and allowed to polymerize. Once set, a comb is removed to create wells for sample loading, and the gel is placed into an electrophoresis chamber with a running buffer.