How to calculate absorbance using the Beer-Lambert Law?
How to calculate absorbance using the Beer-Lambert Law?
Answered
Absorbance (A) is calculated using the Beer-Lambert Law, represented by the formula A = εlc. Here, ε is the molar absorptivity of the analyte at the chosen wavelength (measured in L·mol⁻¹·cm⁻¹), which indicates how strongly the substance absorbs light at that wavelength. l is the path length of the sample, typically measured in centimeters, which refers to the distance the light travels through the solution. c is the concentration of the analyte in the solution, usually in mol/L. To calculate absorbance, simply multiply these three values together: the molar absorptivity, the path length of the solution, and the concentration of the absorbing species. The resulting absorbance value is a dimensionless quantity, and it will indicate how much light is absorbed by the solution at the specific wavelength.
BNO Team