Sourav PanFebruary 9, 2025

how does polyacrylamide gel electrophoresis differ from agarose gel

Sourav PanFebruary 9, 2025

Answered

The primary difference between polyacrylamide and agarose gel electrophoresis lies in their gel composition and applications. Polyacrylamide gels have smaller pores and are used for high-resolution separation of proteins and small nucleic acids, whereas agarose gels have larger pores and are mainly used for DNA and RNA separation. Additionally, polyacrylamide gels require polymerization using acrylamide and bis-acrylamide, while agarose gels are simply melted and cooled to form a matrix.

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