How does high throughput sequencing work?
How does high throughput sequencing work?
Answer
High-throughput sequencing involves several key steps. First, DNA is fragmented into smaller pieces, and these fragments are then attached to a solid surface or a bead. Next, the fragments are amplified to create clusters or libraries of identical DNA sequences. During the sequencing phase, each fragment is sequenced simultaneously using various technologies, such as sequencing by synthesis, where fluorescently labeled nucleotides are incorporated into the growing DNA strand. The emitted signals are detected and recorded to determine the DNA sequence. Finally, computational algorithms are used to assemble the short sequences into longer, contiguous sequences and to analyze the resulting data. This process allows for the rapid and efficient sequencing of large amounts of DNA.
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