How can it be verified that an animal is a transgenic organism?

Verification of transgenic status is crucial to ensure that the genetic modifications have been successfully incorporated. This process often involves molecular techniques such as Polymerase Chain Reaction (PCR), which amplifies specific DNA sequences to detect the presence of the transgene. In addition to PCR, Southern blotting may be employed to analyze the integration pattern of the transgene within the genome. Moreover, expression assays can be conducted to assess whether the transgene is being expressed at the mRNA and protein levels. Overall, a combination of these molecular techniques provides a robust framework for confirming the transgenic nature of an organism.