IB Biology 10 Views 1 Answers
How can examples of DNA profiles be analyzed for comparison?
How can examples of DNA profiles be analyzed for comparison?
Answered
DNA profiling is a powerful tool used in forensic science and paternity testing to analyze genetic material for comparison between samples. The process involves several key techniques and methodologies that allow for the identification of unique DNA patterns. Here’s how DNA profiles can be analyzed for comparison:
Methods of DNA Profiling Analysis
- Sample Preparation:
- DNA is extracted from biological samples such as blood, saliva, or hair. This extraction process isolates the DNA from other cellular materials, ensuring that the sample is suitable for analysis.
- Amplification:
- The Polymerase Chain Reaction (PCR) is commonly used to amplify specific regions of DNA, particularly short tandem repeats (STRs). PCR allows for the creation of millions of copies of targeted DNA sequences, making it possible to analyze even small or degraded samples.
- STR Analysis:
- STR analysis is the primary method used in forensic DNA profiling today. It focuses on specific loci in the genome where short sequences of DNA are repeated. The number of repeats can vary between individuals, providing a unique genetic fingerprint.
- Typically, 13 core STR loci are analyzed in forensic cases, as established by the FBI, allowing for high discrimination power when comparing profiles.
- Electrophoresis:
- After amplification, the DNA fragments are separated by size using gel electrophoresis or capillary electrophoresis. This technique allows for visualization of the amplified STRs, where smaller fragments migrate faster through the gel than larger ones.
- Comparison of Profiles:
- The resulting DNA profiles from different samples are compared by examining the allele patterns at each STR locus. A match at all loci indicates a high probability that the samples originate from the same individual, while mismatches can exclude individuals as potential sources.
- For example, if a crime scene sample matches a suspect’s profile at all 13 STR loci, statistical analysis is performed to determine how rare that specific profile is within a given population. This involves calculating allele frequencies and using population genetics principles like Hardy-Weinberg equilibrium to estimate the likelihood of such a match occurring by chance.
- Statistical Analysis:
- Once matches are established, statistical calculations are conducted to assess the significance of the match. This includes determining the frequency of each allele in relevant population groups and calculating an overall profile frequency. For instance, if a suspect’s profile has a 1 in 1.5 billion chance of matching randomly with another individual in their ethnic group, this provides strong evidence linking them to the sample.
- Interpretation and Reporting:
- Finally, forensic analysts interpret the results and prepare reports summarizing their findings. These reports include details about the methods used, results obtained, and statistical significance of matches or exclusions.
Did this page help you?