Biotechnology 2 Views 1 Answers
HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following: I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine. II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme. Options: (A) only I is true (B) only II is true (C) both I and II are true (D) I is true and II is false
HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following:
I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine.
II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme.
Options:
(A) only I is true
(B) only II is true
(C) both I and II are true
(D) I is true and II is false
Answer
Answer: (C) both I and II are true
Explanation: Hybridomas grow in the presence of HAT because they inherit HGPRT genes from B-cells allowing them to utilize hypoxanthine. Myeloma cells, lacking HGPRT, cannot grow due to aminopterin’s blocking effect on de novo synthesis.
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