Explain the principle and applications of each of the following biochemical techniques: (i) Colorimetry.
Explain the principle and applications of each of the following biochemical techniques:
(i) Colorimetry.
Answer
Colorimetry: Measures the concentration of a substance in solution by measuring the intensity of color. The principle is based on the Beer-Lambert law, which states that absorbance is proportional to concentration.
Applications: Used in biochemical assays to quantify the presence of specific compounds, such as proteins, nucleic acids, and metabolites.