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Sourav PanAugust 20, 2024

Explain the method of DNA sequencing using automated DNA sequencing technique.

Explain the method of DNA sequencing using automated DNA sequencing technique.

Sourav Pan
Sourav PanAugust 20, 2024

Answered

Automated DNA Sequencing Technique:
1. Preparation: DNA is fragmented and each fragment is amplified.
2. Labeling: Fluorescently labeled dideoxynucleotides (ddNTPs) are used to terminate the DNA chain at different points.
3. Separation: The fragments are separated by size using capillary electrophoresis.
4. Detection: A laser detects the fluorescent tags on the ddNTPs as the fragments pass through a detector, and the sequence is read by a computer.

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