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Explain the method of DNA sequencing using automated DNA sequencing technique.
Explain the method of DNA sequencing using automated DNA sequencing technique.
Answer
Automated DNA Sequencing Technique:
1. Preparation: DNA is fragmented and each fragment is amplified.
2. Labeling: Fluorescently labeled dideoxynucleotides (ddNTPs) are used to terminate the DNA chain at different points.
3. Separation: The fragments are separated by size using capillary electrophoresis.
4. Detection: A laser detects the fluorescent tags on the ddNTPs as the fragments pass through a detector, and the sequence is read by a computer.
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