what is the name of the kinetic intermediate in protein folding where secondary structure elements have formed their regular H-bonding patterns and there has been collapse to separate most nonpolar side chains into the protein interior and most hydrophilic side chains to the protein exterior, but the side chain packing has not yet been optimized to achieve a tightly packed protein interior with a crystalline density?
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1. If you did “overload” your Ni+2 Agarose column with a GCE sample that contained too much rGFP, would you expect to see a band in the W2 lane of the Western Blot? If so what is it’s MW? Would you expect to see a band in the E2 lane? If so what is it’s MW?
2. Chromatography columns have a limited protein binding capacity. During the Ni2+ agarose column lab, you shouldnt have overloaded the column. If you did the W2 fraction may have fluoresced slightly. If you see a band in the Western Blot W2 lane, what does this data say about the physical protein structure of rGFP in that lane? Give a possible MW for this band based upon the data.
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In
an erythrocyte undergoing glycolysis, what would be the effect of a
sudden increase in the concentration of
a. ATP? b. AMP?
c. fructose-1,6-bisphosphate? d. fructose-2,6-bisphosphate?
e. citrate? f. glucose-6-phosphate?
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1. Briefly explain why you should not heat organic solvents over a Bunsen burner flame.
2. Indicate a procedure to solve the following recrystallization problems.
(a) oiling out
(b) lack of crystal formation
(c) presence of colored impurities
3. Compound A, a white crystalline solid with a melting point of 75°C, has the solubility profile shown
in the following table. Which of the solvents listed would be a good recrystallizing solvent for
Compound A? Briefly explain.
Compound A — solubility profile
solvent solvent boiling pt. solubility at 25°C solubility at boiling point
water 100. c insol. sol.
methanol 65°C insol. sol.
acetone 56°C sol. sol.
diethyl ether 35°C sol. sol.
4. A student purified a 500. mg sample of phthalic acid by recrystallization from water. The published
solubility of phthalic acid in 100. mL of water is 0.54 g at 14°C and 18 g at 100.°C. You must show your
calculations for the questions below.
(a) What is the smallest volume of boiling water the student could use to dissolve 500. mg of phthalic
acid?
(b) Dissolution of phthalic acid in boiling water produced a clear solution. The student allowed the
solution to cool, then put the solution in an ice bath. Crystals formed, and the student isolated 380 mg of
phthalic acid. Calculate the percent recovery of phthalic acid in this experiment.
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which cells resemble basophils and are found in connective tissue close to small blood vessels
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outline 2 properties of plasma membrane
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rolling circle model of dna replication
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The construction of Parenchyma cells
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how do woman get in their period
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quelq est le principe de la méthode elisa sandwich?
What is the principle of the ELISA sandwich method?
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