What is the Henderson-Hasselbalch equation used for?
What is the Henderson-Hasselbalch equation used for?

What is the Henderson-Hasselbalch equation used for?
What is the Henderson-Hasselbalch equation?
Can you use moles in the Henderson-Hasselbalch equation?
How to rearrange the Henderson-Hasselbalch equation?
How to use the Henderson-Hasselbalch equation to find pH?
How to use the Henderson-Hasselbalch equation to find concentration?
What does the Henderson-Hasselbalch equation tell us?
1. If you did “overload” your Ni+2 Agarose column with a GCE sample that contained too much rGFP, would you expect to see a band in the W2 lane of the Western Blot? If so what is it’s MW? Would you expect to see a band in the E2 lane? If so what is it’s MW?
2. Chromatography columns have a limited protein binding capacity. During the Ni2+ agarose column lab, you shouldnt have overloaded the column. If you did the W2 fraction may have fluoresced slightly. If you see a band in the Western Blot W2 lane, what does this data say about the physical protein structure of rGFP in that lane? Give a possible MW for this band based upon the data.
In
an erythrocyte undergoing glycolysis, what would be the effect of a
sudden increase in the concentration of
a. ATP? b. AMP?
c. fructose-1,6-bisphosphate? d. fructose-2,6-bisphosphate?
e. citrate? f. glucose-6-phosphate?
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