Questions and Answers

A mutant Gα protein with increased GTPase activity would
(A) not bind to GTP
(B) not bind to GDP
(C) show increased signaling
(D) show decreased signaling

Match Group I with Group II
Group I:
P. Staphylococcus aureus
Q. Candida albicans
R. Mycobacterium tuberculosis
S. Lactobacillus lactis
Group II:
1. Biofilms
2. Bacteriocins
3. Methicillin resistance
4. Isoniazid
(A) P-1, Q-4, R-2, S-3
(B) P-2, Q-3, R-1, S-4
(C) P-3, Q-1, R-4, S-2
(D) P-1, Q-2, R-4, S-3

Match the items in Group I with Group II
Group I (Vectors):
P. λ phage
Q. Bacterial Artificial Chromosomes (BACs)
R. P1 derived Artificial Chromosomes (PACs)
S. λ cosmid
Group II (Maximum DNA packaging):
1. 35-45 kb
2. 100-300 kb
3. ≤ 300 kb
4. 5 – 25 kb
(A) P-3, Q-4, R-1, S-2
(B) P-1, Q-3, R-2, S-4
(C) P-4, Q-3, R-2, S-1
(D) P-1, Q-2, R-3, S-4

The turnover numbers for the enzymes, E1 and E2 are 150 s⁻¹ and 15 s⁻¹ respectively. This means
(A) E1 binds to its substrate with higher affinity than E2
(B) The velocity of reactions catalyzed by E1 and E2 at their respective saturating substrate concentrations could be equal, if concentration of E2 used is 10 times that of E1
(C) The velocity of E1 catalyzed reaction is always greater than that of E2
(D) The velocity of E1 catalyzed reaction at a particular enzyme concentration and saturating substrate concentration is lower than that of E2 catalyzed reaction under the same conditions

Consider the following statements.
I. T4 DNA ligase can catalyze blunt end ligation more efficiently than E.coli DNA ligase
II. The ligation efficiency of T4 DNA ligase can be increased with PEG and ficoll.
(A) only I is true
(B) both I and II are true
(C) only II is true
(D) I is true and II is false

Match the promoters listed in Group I with the tissues listed in Group II
Group I:
P. α-Amylase
Q. Glutenin
R. Phaseollin
S. Patatin
Group II:
1. Endosperm
2. Tuber
3. Aleurone
4. Cotyledon
(A) P-3, Q-1, R-4, S-2
(B) P-3, Q-4, R-1, S-2
(C) P-4, Q-2, R-1, S-3
(D) P-1, Q-3, R-2, S-4

Match Group I with Group II
Group I:
P. Fibronectin
Q. Insulin
R. α-Macroglobulin
S. Transferrin
Group II:
1. Uptake of amino acids and glucose
2. Trypsin inhibitor
3. Binds iron
4. Cell attachment to substratum
(A) P-2, Q-1, R-4, S-3
(B) P-3, Q-2, R-1, S-4
(C) P-4, Q-2, R-1, S-3
(D) P-4, Q-1, R-2, S-3

Match the chemicals in Group I with the possible type/class in Group II
Group I:
P. Picloram
Q. Zeatin
R. Thiamine
S. Glutamine
Group II:
1. Vitamin
2. Auxin
3. Amino Acid
4. Cytokinin
(A) P-2, Q-4, R-1, S-3
(B) P-4, Q-1, R-2, S-3
(C) P-3, Q-1, R-2, S-4
(D) P-4, Q-2, R-1, S-3

Match the following antibiotics in Group I with their mode of action in Group II
Group I:
P. Chloramphenicol
Q. Norfloxacin
R. Puromycin
S. Rifampicin
Group II:
1. Binds to DNA gyrase
2. Binds to RNA Polymerase
3. Inhibits peptidyl transferase
4. Mimics aminoacyl-(tRNA**
(A) P-1, Q-3, R-2, S-4
(B) P-3, Q-1, R-2, S-4
(C) P-3, Q-1, R-4, S-2
(D) P-4, Q-2, R-3, S-1

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r)
Assertion: MTT assay is used to determine cell viability based on the principle of colour formation by DNA fragmentation.
Reason: MTT assay is used to determine cell viability based on the colour development by converting tetrazolium soluble salt to insoluble salt.
(A) both (a) and (r) are true and (r) is the correct reason for (a)
(B) both (a) and (r) are true and (r) is not the correct reason for (a)
(C) (a) is true but (r) is false
(D) (a) is false but (r) is true