Questions and Answers

The turnover numbers for the enzymes, E1 and E2 are 150 s⁻¹ and 15 s⁻¹ respectively. This means
(A) E1 binds to its substrate with higher affinity than E2
(B) The velocity of reactions catalyzed by E1 and E2 at their respective saturating substrate concentrations could be equal, if concentration of E2 used is 10 times that of E1
(C) The velocity of E1 catalyzed reaction is always greater than that of E2
(D) The velocity of E1 catalyzed reaction at a particular enzyme concentration and saturating substrate concentration is lower than that of E2 catalyzed reaction under the same conditions

Consider the following statements.
I. T4 DNA ligase can catalyze blunt end ligation more efficiently than E.coli DNA ligase
II. The ligation efficiency of T4 DNA ligase can be increased with PEG and ficoll.
(A) only I is true
(B) both I and II are true
(C) only II is true
(D) I is true and II is false

Match the promoters listed in Group I with the tissues listed in Group II
Group I:
P. α-Amylase
Q. Glutenin
R. Phaseollin
S. Patatin
Group II:
1. Endosperm
2. Tuber
3. Aleurone
4. Cotyledon
(A) P-3, Q-1, R-4, S-2
(B) P-3, Q-4, R-1, S-2
(C) P-4, Q-2, R-1, S-3
(D) P-1, Q-3, R-2, S-4

Match Group I with Group II
Group I:
P. Fibronectin
Q. Insulin
R. α-Macroglobulin
S. Transferrin
Group II:
1. Uptake of amino acids and glucose
2. Trypsin inhibitor
3. Binds iron
4. Cell attachment to substratum
(A) P-2, Q-1, R-4, S-3
(B) P-3, Q-2, R-1, S-4
(C) P-4, Q-2, R-1, S-3
(D) P-4, Q-1, R-2, S-3

Match the chemicals in Group I with the possible type/class in Group II
Group I:
P. Picloram
Q. Zeatin
R. Thiamine
S. Glutamine
Group II:
1. Vitamin
2. Auxin
3. Amino Acid
4. Cytokinin
(A) P-2, Q-4, R-1, S-3
(B) P-4, Q-1, R-2, S-3
(C) P-3, Q-1, R-2, S-4
(D) P-4, Q-2, R-1, S-3

Match the following antibiotics in Group I with their mode of action in Group II
Group I:
P. Chloramphenicol
Q. Norfloxacin
R. Puromycin
S. Rifampicin
Group II:
1. Binds to DNA gyrase
2. Binds to RNA Polymerase
3. Inhibits peptidyl transferase
4. Mimics aminoacyl-(tRNA**
(A) P-1, Q-3, R-2, S-4
(B) P-3, Q-1, R-2, S-4
(C) P-3, Q-1, R-4, S-2
(D) P-4, Q-2, R-3, S-1

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r)
Assertion: MTT assay is used to determine cell viability based on the principle of colour formation by DNA fragmentation.
Reason: MTT assay is used to determine cell viability based on the colour development by converting tetrazolium soluble salt to insoluble salt.
(A) both (a) and (r) are true and (r) is the correct reason for (a)
(B) both (a) and (r) are true and (r) is not the correct reason for (a)
(C) (a) is true but (r) is false
(D) (a) is false but (r) is true

A roller bottle culture vessel perfectly cylindrical in shape having inner radius (r) = 10 cm and length (l) = 20 cm was fitted with a spiral film of length (L) = 30 cm and width (W) = 20 cm. If the film can support 10^5 anchorage dependent cells per cm^2, the increase in the surface area after fitting the spiral film and the additional number of cells that can be grown respectively are
(A) 1200 cm^2 and 12 x 10^7 cells
(B) 600 cm^2 and 6 x 10^7 cells
(C) 600 cm^2 and 8300 cells
(D) 1200 cm^2 and 8300 cells

An immobilized enzyme being used in a continuous plug flow reactor exhibits an effectiveness factor (η) of 1.2. The value of η being greater than 1 could be apparently due to
(A) substrate inhibited kinetics with external pore diffusion limitation
(B) internal pore diffusion limitation
(C) sigmoidal kinetics
(D) unstability of the enzyme

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r):
Assertion: Somatic embryogenesis in plants is a two-step process comprising of embryo initiation followed by embryo production.
Reason: Embryo initiation is independent of the presence of 2,4-dichlorophenoxyacetic acid whereas embryo production requires a high concentration of 2,4-dichlorophenoxyacetic acid.
(A) both (a) and (r) are true and (r) is the correct reason for (a)
(B) both (a) and (r) are true and (r) is not the correct reason for (a)
(C) (a) is true but (r) is false
(D) (a) is false but (r) is true