Questions and Answers

Q.8 During transcription
(A) DNA Gyrase introduces negative supercoils and DNA Topoisomerase I removes negative supercoils
(B) DNA Topoisomerase I introduces negative supercoils and DNA Gyrase removes negative supercoils
(C) both DNA Gyrase and DNA Topoisomerase I introduce negative supercoils
(D) both DNA Gyrase and DNA Topoisomerase I remove negative supercoils

Q.7 In transgenics, alterations in the sequence of nucleotide in genes are due to
P. Substitution
Q. Deletion
R. Insertion
S. Rearrangement
(A) P and Q
(B) P, Q and R
(C) Q and R
(D) R and S

Q.6 Peptidyl transferase activity resides in
(A) 16S rRNA
(B) 23S rRNA
(C) 5S rRNA
(D) 28S rRNA

Q.5 Program used for essentially local similarity search is
(A) BLAST
(B) RasMol
(C) ExPASY
(D) SWISS-PROT

Q.4 Antibiotic resistance marker that CANNOT be used in a cloning vector in Gram negative bacteria is
(A) Streptomycin
(B) Ampicillin
(C) Vancomycin
(D) Kanamycin

Q.3 The bacteria known to be naturally competent for transformation of DNA is
(A) Escherichia coli
(B) Bacillus subtilis
(C) Mycobacterium tuberculosis
(D) Yersinia pestis

Q.2 Ames test is used to determine
(A) the mutagenicity of a chemical
(B) carcinogenicity of a chemical
(C) both mutagenicity and carcinogenicity of a chemical
(D) toxicity of a chemical

Q.1 Hybridoma technology is used to produce
(A) monoclonal antibodies
(B) polyclonal antibodies
(C) both monoclonal and polyclonal antibodies
(D) B cells