HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following:
I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine.
II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme.
Options:
(A) only I is true
(B) only II is true
(C) both I and II are true
(D) I is true and II is false
1 Answer
Value of the determinant mentioned below is:
Matrix=1402101101−12−121 Options:
(A) 24
(B) -30
(C) -24
(D) -10
1 Answer
Identify the CORRECT statements:
P. 5′ and 3′ ends of the transcripts can be mapped by utilizing polymerase chain reaction
Q. S1 nuclease can cleave the DNA strand of a DNA-RNA hybrid
R. T4 polynucleotide kinase is used for labeling 3′ end of DNA
S. Baculovirus (Autographa californica) can be used as an insect expression vector
Options:
(A) P and Q only
(B) R and S only
(C) P and S only
(D) Q and R only
1 Answer
Maximum specific growth rate (Mmax) of a microorganism is calculated by taking the:
(In=loge, X=biomass, t = time)
Options:
(A) slope of In X vs t of the growth cycle
(B) slope of In X vs t during the exponential growth phase
(C) slope of X vs t
(D) slope of X vs t during the exponential phase of growth
1 Answer
Which of the following characteristics with respect to bacterial DNA polymerase are TRUE?
P. Initiation of chain synthesis
Q. 5′-3′ polymerization
R. 3′-5′ exonuclease activity
S. 5′-3′ exonuclease activity
Options:
(A) P and Q only
(B) Q and R only
(C) Q and S only
(D) P and S only
1 Answer
Determine the correctness or otherwise of the following Assertion (a) and the Reason (r):
Assertion: In synchronous culture, majority of the cells move to next phase of the cell cycle simultaneously.
Reason: Synchronous culture could be obtained by starving cells for essential nutrient components.
Options:
(A) both (a) and (r) are true and (r) is the correct reason for (a)
(B) both (a) and (r) are true but (r) is not the correct reason for (a)
(C) (a) is true but (r) is false
(D) (a) is false but (r) is true
1 Answer
Determine the correctness or otherwise of the following Assertion (a) and the Reason (r):
Assertion: Isopropylthiogalactoside (IPTG) is a gratuitous inducer of lactose operon.
Reason: Gratuitous inducers are chemical analogs which behave like natural inducers but they do not serve as substrate for the enzymes that are subsequently synthesized.
Options:
(A) both (a) and (r) are true and (r) is the correct reason for (a)
(B) both (a) and (r) are true but (r) is not the correct reason for (a)
(C) (a) is true but (r) is false
(D) (a) is false but (r) is true
1 Answer
Protein-protein interactions are studied by:
P. DNA foot printing
Q. Yeast two hybrid system
R. Ligase chain reaction
S. Mass spectrometry
Options:
(A) P and S only
(B) Q and S only
(C) P and R only
(D) Q and R only
1 Answer
Match the terms in Group I with their associated functions in Group II:
Group I
P. Shine-Dalgarno sequences
Q. Leucine zipper
R. Aminoacyl tRNA synthetase
S. RNA interference (RNAi)
Group II
1. Aminoacylation of tRNA
2. Gene silencing
3. Ribosome binding and facilitation of translation initiation
4. Transcription factors
Options:
(A) P-3, Q-4, R-1, S-2
(B) P-4, Q-3, R-2, S-1
(C) P-2, Q-3, R-1, S-4
(D) P-3, Q-2, R-4, S-1
1 Answer
Determine the correctness or otherwise of the following Assertion (a) and the Reason (r):
Assertion: A very low amount of inhibitor can act as an activator for allosteric enzymes.
Reason: Allosteric enzymes follow Michaelis-Menten kinetics.
Options:
(A) both (a) and (r) are true and (r) is the correct reason for (a)
(B) both (a) and (r) are true but (r) is not the correct reason for (a)
(C) (a) is true but (r) is false
(D) (a) is false but (r) is true
1 Answer
