Which one of the following is the correct match between the molecular properties listed in Column I and the corresponding biochemical separation methods in Column II?

(A) P-i; Q-ii; R-v; S-iii
(B) P-iii; Q-iv; R-ii; S-i
(C) P-iii; Q-iv; R-i; S-ii
(D) P-v; Q-iv; R-iii; S-ii

Metabolic intermediates provide the backbone for the synthesis of amino acids. Match the metabolic intermediates listed in Column I with their corresponding amino acids given in Column II.

(A) P-ii; Q-i; R-v; S-iv
(B) P-iii; Q-ii; R-i; S-v
(C) P-iv; Q-iii; R-ii; S-v
(D) P-ii; Q-i; R-iv; S-v

If a 10 mM solution of a biomolecule in a cuvette of path length 10 mm absorbs 90% of the incident light at 280 nm, the molar extinction coefficient of the biomolecule at this wavelength is _____________ M⁻¹cm⁻¹.

In a lactic acid solution at pH 4.8, the concentrations of lactic acid and lactate are 0.01 M and 0.087 M, respectively. The calculated pKa of lactic acid is ________.

A newly identified viral protein contains one long α-helix spanning 60 amino acid residues. The number of main chain H-bonds formed in this helix is __________.

Which one or more of the following statements correctly describe(s) the addition of N-nucleotides during the rearrangement of the immunoglobulin heavy chain-encoding gene?

(A) Addition of N-nucleotides is template encoded.
(B) N-nucleotides are added by terminal deoxynucleotidyl transferase.
(C) The added N-nucleotides are common in V-D and D-J junction.
(D) N-nucleotides are added by the DNA polymerase II.

In mammals, hematopoietic stem cells that give rise to different types of blood cells are known as

(A) totipotent stem cells.
(B) pluripotent stem cells.
(C) myeloid progenitor cells.
(D) lymphoid progenitor cells.

Which one of the following metabolic intermediates is common to glycolysis, nucleotide synthesis, and glycogen synthesis?

(A) Citrate
(B) Oxaloacetate
(C) Glucose 6-phosphate
(D) Glycerol 3-phosphate

Mammalian cells cultured at low temperature (25 to 30°C) lead to an increased sterol content in the membrane. Elevated sterols in the membrane result in

(A) an enhancement in membrane fluidity.
(B) stabilization of membrane proteins.
(C) an increase in membrane permeability to water.
(D) a decrease in membrane fluidity.

Which one of the following pairs of amino acids is NOT incorporated in a polypeptide chain?

(A) 4-Hydroxyproline and γ-carboxyglutamate
(B) γ-Carboxyglutamate and desmosine
(C) Ornithine and citrulline
(D) 4-Hydroxyproline and 5-hydroxylysine