Questions and Answers

Which statement about DNA sequencing is false? a. The method developed by Sanger in the 1970s involved chemical modifications of bases b. The method developed by Sanger in the 1970s involved breaking up the DNA molecule into numerous small fragments. C_ High-throughput sequencing is generally faster than Sanger sequencing: d_ none of these statements are false

Which is part of a DNA sequencing technique that is useful for high-throughput sequencing, but is not part of Sanger DNA sequencing?

Select one:

a. Using dideoxynucleotides as chain terminators

b. Removal of a chemical group that blocks the 3′ end of the new DNA strand

c. Labeling deoxynucleotides with fluorescent tags

d. Separating DNA by size using gel electrophoresis

e. Hybridization between the template and a primer