Questions and Answers

What DNA sequencing technique is useful for high throughput sequencing?

Which of the following is a mechanism used in next generation high throughput DNA sequencing?

What is the output of high-throughput sequencing?

Which of the following about Next Generation Sequencing is true?

Does not require primers

Employs RNA polymerase

Individual DNA fragments are amplified on a solid support Is not high throughput

Next Generation Sequencing is an alternative term for Sanger sequencing

The neurotoxin diisopropylphosphofluoridate (DIPF) binds only to a serine amino acid residue at the active site of acetylcholinesterase to inhibit the enzyme. What type of inhibitor is it?

Which of the following is a mechanism used in next generation high throughput DNA sequencing?

-Ion Torrent

-3 Step Method

-Chain Terminators

-Sanger Sequencing

Which statement about DNA sequencing is false? a. The method developed by Sanger in the 1970s involved chemical modifications of bases b. The method developed by Sanger in the 1970s involved breaking up the DNA molecule into numerous small fragments. C_ High-throughput sequencing is generally faster than Sanger sequencing: d_ none of these statements are false

Which is part of a DNA sequencing technique that is useful for high-throughput sequencing, but is not part of Sanger DNA sequencing?

Select one:

a. Using dideoxynucleotides as chain terminators

b. Removal of a chemical group that blocks the 3′ end of the new DNA strand

c. Labeling deoxynucleotides with fluorescent tags

d. Separating DNA by size using gel electrophoresis

e. Hybridization between the template and a primer