Explain that in non-cyclic photophosphorylation: • photosystem I (PSI) and photosystem II (PSII) are both involved • photoactivation of chlorophyll occurs • the oxygen-evolving complex catalyses the photolysis of water • ATP and reduced NADP are synthesised
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Explain that in cyclic photophosphorylation: • only photosystem I (PSI) is involved • photoactivation of chlorophyll occurs • ATP is synthesised
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State that cyclic photophosphorylation and non-cyclic photophosphorylation occur during the light-dependent stage of photosynthesis
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Describe and use chromatography to separate and identify chloroplast pigments (reference should be made to Rf values in identification of chloroplast pigments)
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Interpret absorption spectra of chloroplast pigments and action spectra for photosynthesis
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Describe the role of chloroplast pigments (chlorophyll a, chlorophyll b, carotene and xanthophyll) in light absorption in thylakoids
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State that within a chloroplast, the thylakoids (thylakoid membranes and thylakoid spaces), which occur in stacks called grana, are the site of the light-dependent stage and the stroma is the site of the light-independent stage
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Explain that energy transferred as ATP and reduced NADP from the light-dependent stage is used during the lightindependent stage (Calvin cycle) of photosynthesis to produce complex organic molecules
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Describe and carry out investigations using simple respirometers to determine the effect of temperature on the rate of respiration
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Describe and carry out investigations using redox indicators, including DCPIP and methylene blue, to determine the effects of temperature and substrate concentration on the rate of respiration of yeast
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