State that cyclic photophosphorylation and non-cyclic photophosphorylation occur during the light-dependent stage of photosynthesis

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Describe and use chromatography to separate and identify chloroplast pigments (reference should be made to Rf values in identification of chloroplast pigments)

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Interpret absorption spectra of chloroplast pigments and action spectra for photosynthesis

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Describe the role of chloroplast pigments (chlorophyll a, chlorophyll b, carotene and xanthophyll) in light absorption in thylakoids

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State that within a chloroplast, the thylakoids (thylakoid membranes and thylakoid spaces), which occur in stacks called grana, are the site of the light-dependent stage and the stroma is the site of the light-independent stage

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Explain that energy transferred as ATP and reduced NADP from the light-dependent stage is used during the lightindependent stage (Calvin cycle) of photosynthesis to produce complex organic molecules

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Describe and carry out investigations using simple respirometers to determine the effect of temperature on the rate of respiration

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Describe and carry out investigations using redox indicators, including DCPIP and methylene blue, to determine the effects of temperature and substrate concentration on the rate of respiration of yeast

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Explain how rice is adapted to grow with its roots submerged in water, limited to the development of aerenchyma in roots, ethanol fermentation in roots and faster growth of stems

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Explain why the energy yield from respiration in aerobic conditions is much greater than the energy yield from respiration in anaerobic conditions (a detailed account of the total yield of ATP from the aerobic respiration of glucose is not expected)

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