Viability Staining

Cell Viability test using Propidium Iodide (PI) – Principle, Procedure, uses

• • 9 min readby
Procedure of Viability test by propidium iodide

Cell viability analysis using Propidium Iodide is a simple method in which the dye is used to distinguish between living and dead cells. It is the process where Propidium Iodide (PI) is added to a cell suspension and the stained cells is then examined usually by flow cytometer. PI is a red-fluorescent dye that binds … Read more

Viability Staining by Loeffler’s Method – Principle, Procedure, Uses

• • 11 min readby
Procedure of Viability Staining by Loeffler’s Method

Loeffler’s methylene blue staining is a simple staining method used mainly for observing the morphology of Corynebacterium diphtheriae. It is the process where Loeffler’s alkaline methylene blue solution is applied on a fixed smear, and this stain contains a small amount of potassium hydroxide (KOH) which makes the solution alkaline. It is the alkalinity that … Read more