Gel Electrophoresis buffer

Different Buffer preparation for Gel Electrophoresis

4 min readby
Buffer preparation

Acrylamide Stock Solution Preparation Application: Acrylamide is used along with bisacrylamide to form gels used in SDS-PAGE. Acrylamide forms polyacrylamide polymers, while bisacrylamide crosslinks between polyacrylamide chains. To prepare 1L of Acrylamide Stock Solution the following components are required; Component Amount Concentration acrylamide (mw: 71.08 g/mol) 389.6 g 5.481 M bisacrylamide (mw: 154.17 g/mol) 10.4 … Read more

Ad Blocker Detected
We've detected that you're using an ad blocker. Some content may not display properly.
Why are you seeing this? Ad blockers can prevent certain content and features from loading correctly on our website.

To continue with the best experience:

We respect your choice to use ad blockers. This message helps ensure you get the full experience.