Difference Between Brightfield and Phase-contrast Microscopy

14 Difference Between Brightfield and Phase-contrast Microscope Topic Brightfield Microscope Phase Contrast Microscope Invented by Antonie van Leeuwenhoek (1632–1724) Frits Zernike (1888-1966) in 1932 Staining The Specimen Must be Stained before their observation under bright field Microscope. Staining is not required. Changes in refractive index This Microscope has no ability to alter the refractive index. … Read more