BCYE Agar

In 1978, Feeley et al developed a medium for isolating Legionella species. They later modified it by replacing casein hydrolysate with beef extract and starch with activated carbon and naming it Charcoal Yeast extract (CYE) Agar. A further modification was made by Pasculle et al in 1980 by the addition of ACES (N-2-acetamido-2-aminoethane sulfonic acid) buffer in order to maintain the proper pH for optimal growth designated as BCYE for Buffered Charcoal Yeast Extract. Edelstein et al modified the medium in 1981 by adding potassium salt to alpha-ketoglutaric acids, which increased the medium’s sensitivity. It is used in primary isolation and cultivation of Legionella species. It is recommended to be used in the cultivation and primary isolation of Legionella spp.