What is not true about using high-throughput next-generation sequencing techniques?
What is not true about using high-throughput next-generation sequencing techniques?
Answer
One common misconception about high-throughput next-generation sequencing techniques is that they rely on chain termination methods, similar to those used in Sanger sequencing. However, NGS technologies do not use chain termination; instead, they use different methods such as sequencing by synthesis, sequencing by ligation, or ion semiconductor sequencing. Additionally, high-throughput sequencing does not require the manual handling of each DNA molecule, as it is designed to automate and parallelize the sequencing process to handle large volumes of data efficiently. These methods provide a more comprehensive and cost-effective approach to sequencing compared to traditional techniques.
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