How is the Beer-Lambert Law used in spectroscopy?
How is the Beer-Lambert Law used in spectroscopy?
Answered
In spectroscopy, the Beer-Lambert Law is used extensively to analyze the concentration of substances in a solution based on their absorbance of light. This law allows for quantitative analysis by measuring the absorbance of a sample at a specific wavelength, which is directly related to its concentration. By knowing the molar absorptivity (ε) of the substance and the path length of the sample cell, the Beer-Lambert Law provides a way to determine the concentration of unknown solutions by comparing their absorbance to that of known standards. The law also plays a crucial role in monitoring chemical reactions by tracking the change in absorbance over time, allowing researchers to study reaction kinetics. Additionally, the Beer-Lambert Law is useful in determining the purity of compounds by assessing the characteristics of their absorption spectra and identifying any impurities that might absorb light at the same wavelength.
BNO Team